Info
- Utilizzare la checkbox di selezione a fianco di ciascun documento per attivare le funzionalità di stampa, invio email, download nei formati disponibili del (i) record.
ABC transporters and multidrug resistance [[electronic resource] /] / edited by Ahcène Boumendjel, Jean Boutonnat, Jacques Robert
| ABC transporters and multidrug resistance [[electronic resource] /] / edited by Ahcène Boumendjel, Jean Boutonnat, Jacques Robert |
| Pubbl/distr/stampa | Hoboken, NJ, : John Wiley & Sons, c2009 |
| Descrizione fisica | 1 online resource (475 p.) |
| Disciplina |
572/.696
615.1 |
| Altri autori (Persone) |
BoumendjelAhcène
BoutonnatJean RobertJacques, M.D. |
| Collana | Wiley series in drug discovery and development |
| Soggetto topico |
ATP-binding cassette transporters
Drug resistance in cancer cells |
| ISBN |
1-282-36879-6
9786612368790 0-470-49513-8 0-470-49512-X |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
ABC TRANSPORTERS AND MULTIDRUG RESISTANCE; CONTENTS; PREFACE; CONTRIBUTORS; INTRODUCTION: WHAT IS MULTIDRUG RESISTANCE?; PART I ABC PROTEINS: AN OVERVIEW AND DESCRIPTION OF THE STRUCTURE, GENOME, NORMAL TISSUE EXPRESSION, PHYSIOLOGICAL ASPECT, AND MECHANISM OF ACTION; 1 The P-glycoprotein 170: Just a multidrug resistance protein or a protean molecule?; 2 Multidrug resistance-associated protein (MRP/ABCC proteins); 3 ABCG2: A new challenge in cancer drug resistance
PART II ABC PROTEINS AND ONCOLOGY: EXPRESSION, DETECTION, AND IMPLICATION OF ABC PROTEINS IN HEMATOLOGICAL MALIGNANCIES AND SOLID TUMORS4 Expression, detection, and implication of ABC proteins in acute myeloblastic leukemia; 5 ABC proteins and oncology: Expression, detection, and implication of ABC proteins in solid tumors; PART III ABC PROTEINS AND PATHOGENIC MICROORGANISMS; 6 ABC transporters and resistance to antibiotics; 7 ABC proteins involved in protozoan parasite resistance PART IV MULTIDRUG RESISTANCE (MDR) MODULATION THROUGH INHIBITION OF ABC TRANSPORTERS: DESIGN OF INHIBITORS AND MECHANISM OF ACTION8 Reversal agents for P-glycoprotein-mediated multidrug resistance; 9 Reversal agents of multidrug resistance mediated by multidrug resistance-associated proteins (MRPs); 10 Reversal agents for breast cancer resistance protein (BCRP)-mediated multidrug resistance; 11 Strategies to overcome drug resistance in acute and chronic leukemias; 12 Multidrug resistance reversal in solid tumors PART V BIOLOGICAL AND CLINICAL ASPECTS OF MULTIDRUG RESISTANCE: THE ROLE OF THE TRANSPORTERS AT THE MAIN PROTECTION BARRIERS (ABCB1, ABCC1, ABCC2, ABCG2) ON THE BIOAVAILABILITY OF MANY TYPES OF DRUGS AND MEDICATIONS13 ABC superfamily transporters at the human blood-brain barrier; 14 The role of ABC transporters at the intestinal barrier; 15 Genetic polymorphisms in ABC transporters; PERSPECTIVES; INDEX |
| Record Nr. | UNINA-9910139988203321 |
| Hoboken, NJ, : John Wiley & Sons, c2009 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
ABC transporters and multidrug resistance [[electronic resource] /] / edited by Ahcène Boumendjel, Jean Boutonnat, Jacques Robert
| ABC transporters and multidrug resistance [[electronic resource] /] / edited by Ahcène Boumendjel, Jean Boutonnat, Jacques Robert |
| Pubbl/distr/stampa | Hoboken, NJ, : John Wiley & Sons, c2009 |
| Descrizione fisica | 1 online resource (475 p.) |
| Disciplina |
572/.696
615.1 |
| Altri autori (Persone) |
BoumendjelAhcène
BoutonnatJean RobertJacques, M.D. |
| Collana | Wiley series in drug discovery and development |
| Soggetto topico |
ATP-binding cassette transporters
Drug resistance in cancer cells |
| ISBN |
1-282-36879-6
9786612368790 0-470-49513-8 0-470-49512-X |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
ABC TRANSPORTERS AND MULTIDRUG RESISTANCE; CONTENTS; PREFACE; CONTRIBUTORS; INTRODUCTION: WHAT IS MULTIDRUG RESISTANCE?; PART I ABC PROTEINS: AN OVERVIEW AND DESCRIPTION OF THE STRUCTURE, GENOME, NORMAL TISSUE EXPRESSION, PHYSIOLOGICAL ASPECT, AND MECHANISM OF ACTION; 1 The P-glycoprotein 170: Just a multidrug resistance protein or a protean molecule?; 2 Multidrug resistance-associated protein (MRP/ABCC proteins); 3 ABCG2: A new challenge in cancer drug resistance
PART II ABC PROTEINS AND ONCOLOGY: EXPRESSION, DETECTION, AND IMPLICATION OF ABC PROTEINS IN HEMATOLOGICAL MALIGNANCIES AND SOLID TUMORS4 Expression, detection, and implication of ABC proteins in acute myeloblastic leukemia; 5 ABC proteins and oncology: Expression, detection, and implication of ABC proteins in solid tumors; PART III ABC PROTEINS AND PATHOGENIC MICROORGANISMS; 6 ABC transporters and resistance to antibiotics; 7 ABC proteins involved in protozoan parasite resistance PART IV MULTIDRUG RESISTANCE (MDR) MODULATION THROUGH INHIBITION OF ABC TRANSPORTERS: DESIGN OF INHIBITORS AND MECHANISM OF ACTION8 Reversal agents for P-glycoprotein-mediated multidrug resistance; 9 Reversal agents of multidrug resistance mediated by multidrug resistance-associated proteins (MRPs); 10 Reversal agents for breast cancer resistance protein (BCRP)-mediated multidrug resistance; 11 Strategies to overcome drug resistance in acute and chronic leukemias; 12 Multidrug resistance reversal in solid tumors PART V BIOLOGICAL AND CLINICAL ASPECTS OF MULTIDRUG RESISTANCE: THE ROLE OF THE TRANSPORTERS AT THE MAIN PROTECTION BARRIERS (ABCB1, ABCC1, ABCC2, ABCG2) ON THE BIOAVAILABILITY OF MANY TYPES OF DRUGS AND MEDICATIONS13 ABC superfamily transporters at the human blood-brain barrier; 14 The role of ABC transporters at the intestinal barrier; 15 Genetic polymorphisms in ABC transporters; PERSPECTIVES; INDEX |
| Record Nr. | UNINA-9910830388703321 |
| Hoboken, NJ, : John Wiley & Sons, c2009 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
ABC transporters and multidrug resistance [[electronic resource] /] / edited by Ahcène Boumendjel, Jean Boutonnat, Jacques Robert
| ABC transporters and multidrug resistance [[electronic resource] /] / edited by Ahcène Boumendjel, Jean Boutonnat, Jacques Robert |
| Pubbl/distr/stampa | Hoboken, NJ, : John Wiley & Sons, c2009 |
| Descrizione fisica | 1 online resource (475 p.) |
| Disciplina |
572/.696
615.1 |
| Altri autori (Persone) |
BoumendjelAhcène
BoutonnatJean RobertJacques, M.D. |
| Collana | Wiley series in drug discovery and development |
| Soggetto topico |
ATP-binding cassette transporters
Drug resistance in cancer cells |
| ISBN |
1-282-36879-6
9786612368790 0-470-49513-8 0-470-49512-X |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
ABC TRANSPORTERS AND MULTIDRUG RESISTANCE; CONTENTS; PREFACE; CONTRIBUTORS; INTRODUCTION: WHAT IS MULTIDRUG RESISTANCE?; PART I ABC PROTEINS: AN OVERVIEW AND DESCRIPTION OF THE STRUCTURE, GENOME, NORMAL TISSUE EXPRESSION, PHYSIOLOGICAL ASPECT, AND MECHANISM OF ACTION; 1 The P-glycoprotein 170: Just a multidrug resistance protein or a protean molecule?; 2 Multidrug resistance-associated protein (MRP/ABCC proteins); 3 ABCG2: A new challenge in cancer drug resistance
PART II ABC PROTEINS AND ONCOLOGY: EXPRESSION, DETECTION, AND IMPLICATION OF ABC PROTEINS IN HEMATOLOGICAL MALIGNANCIES AND SOLID TUMORS4 Expression, detection, and implication of ABC proteins in acute myeloblastic leukemia; 5 ABC proteins and oncology: Expression, detection, and implication of ABC proteins in solid tumors; PART III ABC PROTEINS AND PATHOGENIC MICROORGANISMS; 6 ABC transporters and resistance to antibiotics; 7 ABC proteins involved in protozoan parasite resistance PART IV MULTIDRUG RESISTANCE (MDR) MODULATION THROUGH INHIBITION OF ABC TRANSPORTERS: DESIGN OF INHIBITORS AND MECHANISM OF ACTION8 Reversal agents for P-glycoprotein-mediated multidrug resistance; 9 Reversal agents of multidrug resistance mediated by multidrug resistance-associated proteins (MRPs); 10 Reversal agents for breast cancer resistance protein (BCRP)-mediated multidrug resistance; 11 Strategies to overcome drug resistance in acute and chronic leukemias; 12 Multidrug resistance reversal in solid tumors PART V BIOLOGICAL AND CLINICAL ASPECTS OF MULTIDRUG RESISTANCE: THE ROLE OF THE TRANSPORTERS AT THE MAIN PROTECTION BARRIERS (ABCB1, ABCC1, ABCC2, ABCG2) ON THE BIOAVAILABILITY OF MANY TYPES OF DRUGS AND MEDICATIONS13 ABC superfamily transporters at the human blood-brain barrier; 14 The role of ABC transporters at the intestinal barrier; 15 Genetic polymorphisms in ABC transporters; PERSPECTIVES; INDEX |
| Record Nr. | UNINA-9910840786103321 |
| Hoboken, NJ, : John Wiley & Sons, c2009 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Cell membrane nanodomains : from biochemistry to nanoscopy / / edited by Alessandra Cambi, Diane S. Lidke
| Cell membrane nanodomains : from biochemistry to nanoscopy / / edited by Alessandra Cambi, Diane S. Lidke |
| Pubbl/distr/stampa | Boca Raton : , : Taylor & Francis, , [2015] |
| Descrizione fisica | 1 online resource (496 p.) |
| Disciplina | 572/.696 |
| Soggetto topico | Membrane proteins |
| ISBN |
0-429-07585-5
1-4822-0991-8 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Front Cover; Contents; Preface; Acknowledgments; Editors; Contributors; Chapter 1: Giant Unilamellar Vesicles (GUVs) as a Laboratory to Study Mesoscopic Lipid Domains in Membranes; Chapter 2: An Active Basis for the Nanoscopic Organization of Membrane Components in Living Cell Membranes; Chapter 3: Functional Role of Membrane Lipids in EGF Receptor Dynamics and Regulation; Chapter 4: Tetraspanins as Master Organizers of the Plasma Membrane; Chapter 5: B Cell Receptor Signaling; Chapter 6: Imaging the Complexity, Plasticity, and Dynamics of Caveolae
Chapter 7: Membrane Microdomains Enriched in Ceramides : From Generation to FunctionChapter 8: Domains of Phosphoinositides in the Plasma Membrane; Chapter 9: Signaling Phagocytosis : Role of Specialized Lipid Domains; Chapter 10: Fluctuation Spectroscopy Methods for the Analysis of Membrane Processes; Chapter 11: Spatial Intensity Distribution Analysis (SpIDA) : A Method to Probe Membrane Receptor Organization in Intact Cells; Chapter 12: Live-Cell TIRF Imaging of Molecular Assembly and Plasma Membrane Topography; Chapter 13: Laurdan Identifies Different Lipid Membranes in Eukaryotic Cells Chapter 14: Development of Optical Highlighter Fluorescent Proteins and Their Applications in Super-Resolution Fluorescence MicroscopyChapter 15: Targeting Dyes for Biology; Chapter 16: Combined Topography, Recognition, and Fluorescence Measurements on Cells; Chapter 17: Super-Resolution Imaging with Single-Molecule Localization; Chapter 18: Visualization and Resolution in Localization Microscopy; Chapter 19: Molecular Plasma Membrane Dynamics Dissected by STED Nanoscopy and Fluorescence Correlation Spectroscopy (STED-FCS) Chapter 20: Nanophotonic Approaches for Nanoscale Imaging and Single-Molecule Detection at Ultrahigh ConcentrationsBack Cover |
| Record Nr. | UNINA-9910787839803321 |
| Boca Raton : , : Taylor & Francis, , [2015] | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Cell membrane nanodomains : from biochemistry to nanoscopy / / edited by Alessandra Cambi, Diane S. Lidke
| Cell membrane nanodomains : from biochemistry to nanoscopy / / edited by Alessandra Cambi, Diane S. Lidke |
| Pubbl/distr/stampa | Boca Raton : , : Taylor & Francis, , [2015] |
| Descrizione fisica | 1 online resource (496 p.) |
| Disciplina | 572/.696 |
| Soggetto topico | Membrane proteins |
| ISBN |
0-429-07585-5
1-4822-0991-8 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Front Cover; Contents; Preface; Acknowledgments; Editors; Contributors; Chapter 1: Giant Unilamellar Vesicles (GUVs) as a Laboratory to Study Mesoscopic Lipid Domains in Membranes; Chapter 2: An Active Basis for the Nanoscopic Organization of Membrane Components in Living Cell Membranes; Chapter 3: Functional Role of Membrane Lipids in EGF Receptor Dynamics and Regulation; Chapter 4: Tetraspanins as Master Organizers of the Plasma Membrane; Chapter 5: B Cell Receptor Signaling; Chapter 6: Imaging the Complexity, Plasticity, and Dynamics of Caveolae
Chapter 7: Membrane Microdomains Enriched in Ceramides : From Generation to FunctionChapter 8: Domains of Phosphoinositides in the Plasma Membrane; Chapter 9: Signaling Phagocytosis : Role of Specialized Lipid Domains; Chapter 10: Fluctuation Spectroscopy Methods for the Analysis of Membrane Processes; Chapter 11: Spatial Intensity Distribution Analysis (SpIDA) : A Method to Probe Membrane Receptor Organization in Intact Cells; Chapter 12: Live-Cell TIRF Imaging of Molecular Assembly and Plasma Membrane Topography; Chapter 13: Laurdan Identifies Different Lipid Membranes in Eukaryotic Cells Chapter 14: Development of Optical Highlighter Fluorescent Proteins and Their Applications in Super-Resolution Fluorescence MicroscopyChapter 15: Targeting Dyes for Biology; Chapter 16: Combined Topography, Recognition, and Fluorescence Measurements on Cells; Chapter 17: Super-Resolution Imaging with Single-Molecule Localization; Chapter 18: Visualization and Resolution in Localization Microscopy; Chapter 19: Molecular Plasma Membrane Dynamics Dissected by STED Nanoscopy and Fluorescence Correlation Spectroscopy (STED-FCS) Chapter 20: Nanophotonic Approaches for Nanoscale Imaging and Single-Molecule Detection at Ultrahigh ConcentrationsBack Cover |
| Record Nr. | UNINA-9910800188103321 |
| Boca Raton : , : Taylor & Francis, , [2015] | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Cell membrane nanodomains : from biochemistry to nanoscopy / / edited by Alessandra Cambi, Diane S. Lidke
| Cell membrane nanodomains : from biochemistry to nanoscopy / / edited by Alessandra Cambi, Diane S. Lidke |
| Pubbl/distr/stampa | Boca Raton : , : Taylor & Francis, , [2015] |
| Descrizione fisica | 1 online resource (496 p.) |
| Disciplina | 572/.696 |
| Soggetto topico | Membrane proteins |
| ISBN |
0-429-07585-5
1-4822-0991-8 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Front Cover; Contents; Preface; Acknowledgments; Editors; Contributors; Chapter 1: Giant Unilamellar Vesicles (GUVs) as a Laboratory to Study Mesoscopic Lipid Domains in Membranes; Chapter 2: An Active Basis for the Nanoscopic Organization of Membrane Components in Living Cell Membranes; Chapter 3: Functional Role of Membrane Lipids in EGF Receptor Dynamics and Regulation; Chapter 4: Tetraspanins as Master Organizers of the Plasma Membrane; Chapter 5: B Cell Receptor Signaling; Chapter 6: Imaging the Complexity, Plasticity, and Dynamics of Caveolae
Chapter 7: Membrane Microdomains Enriched in Ceramides : From Generation to FunctionChapter 8: Domains of Phosphoinositides in the Plasma Membrane; Chapter 9: Signaling Phagocytosis : Role of Specialized Lipid Domains; Chapter 10: Fluctuation Spectroscopy Methods for the Analysis of Membrane Processes; Chapter 11: Spatial Intensity Distribution Analysis (SpIDA) : A Method to Probe Membrane Receptor Organization in Intact Cells; Chapter 12: Live-Cell TIRF Imaging of Molecular Assembly and Plasma Membrane Topography; Chapter 13: Laurdan Identifies Different Lipid Membranes in Eukaryotic Cells Chapter 14: Development of Optical Highlighter Fluorescent Proteins and Their Applications in Super-Resolution Fluorescence MicroscopyChapter 15: Targeting Dyes for Biology; Chapter 16: Combined Topography, Recognition, and Fluorescence Measurements on Cells; Chapter 17: Super-Resolution Imaging with Single-Molecule Localization; Chapter 18: Visualization and Resolution in Localization Microscopy; Chapter 19: Molecular Plasma Membrane Dynamics Dissected by STED Nanoscopy and Fluorescence Correlation Spectroscopy (STED-FCS) Chapter 20: Nanophotonic Approaches for Nanoscale Imaging and Single-Molecule Detection at Ultrahigh ConcentrationsBack Cover |
| Record Nr. | UNINA-9910827751503321 |
| Boca Raton : , : Taylor & Francis, , [2015] | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Machine learning for protein subcellular localization prediction / / Shibiao Wan, Man-Wai Mak
| Machine learning for protein subcellular localization prediction / / Shibiao Wan, Man-Wai Mak |
| Autore | Wan Shibiao |
| Pubbl/distr/stampa | Berlin, Germany ; ; Boston, Massachusetts : , : De Gruyter, , 2015 |
| Descrizione fisica | 1 online resource (210 p.) |
| Disciplina | 572/.696 |
| Soggetto topico |
Proteins - Physiological transport - Data processing
Machine learning Probabilities - Data processing |
| Soggetto genere / forma | Electronic books. |
| ISBN |
1-5015-0150-X
1-5015-0152-6 |
| Classificazione | WC 7700 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Front matter -- Preface -- Contents -- List of Abbreviations -- 1. Introduction -- 2. Overview of subcellular localization prediction -- 3. Legitimacy of using gene ontology information -- 4. Single-location protein subcellular localization -- 5. From single- to multi-location -- 6. Mining deeper on GO for protein subcellular localization -- 7. Ensemble random projection for large-scale predictions -- 8. Experimental setup -- 9. Results and analysis -- 10. Properties of the proposed predictors -- 11. Conclusions and future directions -- A. Webservers for protein subcellular localization -- B. Support vector machines -- C. Proof of no bias in LOOCV -- D. Derivatives for penalized logistic regression -- Bibliography -- Index |
| Record Nr. | UNINA-9910460442103321 |
Wan Shibiao
|
||
| Berlin, Germany ; ; Boston, Massachusetts : , : De Gruyter, , 2015 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Machine learning for protein subcellular localization prediction / / Shibiao Wan, Man-Wai Mak
| Machine learning for protein subcellular localization prediction / / Shibiao Wan, Man-Wai Mak |
| Autore | Wan Shibiao |
| Pubbl/distr/stampa | Berlin, Germany ; ; Boston, Massachusetts : , : De Gruyter, , 2015 |
| Descrizione fisica | 1 online resource (210 p.) |
| Disciplina | 572/.696 |
| Soggetto topico |
Proteins - Physiological transport - Data processing
Machine learning Probabilities - Data processing |
| Soggetto non controllato |
Bioinformatics
Computer Science Proteomics |
| ISBN |
1-5015-0150-X
1-5015-0152-6 |
| Classificazione | WC 7700 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Front matter -- Preface -- Contents -- List of Abbreviations -- 1. Introduction -- 2. Overview of subcellular localization prediction -- 3. Legitimacy of using gene ontology information -- 4. Single-location protein subcellular localization -- 5. From single- to multi-location -- 6. Mining deeper on GO for protein subcellular localization -- 7. Ensemble random projection for large-scale predictions -- 8. Experimental setup -- 9. Results and analysis -- 10. Properties of the proposed predictors -- 11. Conclusions and future directions -- A. Webservers for protein subcellular localization -- B. Support vector machines -- C. Proof of no bias in LOOCV -- D. Derivatives for penalized logistic regression -- Bibliography -- Index |
| Record Nr. | UNINA-9910797139603321 |
|
Wan Shibiao
|
||
| Berlin, Germany ; ; Boston, Massachusetts : , : De Gruyter, , 2015 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Machine learning for protein subcellular localization prediction / / Shibiao Wan, Man-Wai Mak
| Machine learning for protein subcellular localization prediction / / Shibiao Wan, Man-Wai Mak |
| Autore | Wan Shibiao |
| Pubbl/distr/stampa | Berlin, Germany ; ; Boston, Massachusetts : , : De Gruyter, , 2015 |
| Descrizione fisica | 1 online resource (210 p.) |
| Disciplina | 572/.696 |
| Soggetto topico |
Proteins - Physiological transport - Data processing
Machine learning Probabilities - Data processing |
| Soggetto non controllato |
Bioinformatics
Computer Science Proteomics |
| ISBN |
1-5015-0150-X
1-5015-0152-6 |
| Classificazione | WC 7700 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto | Front matter -- Preface -- Contents -- List of Abbreviations -- 1. Introduction -- 2. Overview of subcellular localization prediction -- 3. Legitimacy of using gene ontology information -- 4. Single-location protein subcellular localization -- 5. From single- to multi-location -- 6. Mining deeper on GO for protein subcellular localization -- 7. Ensemble random projection for large-scale predictions -- 8. Experimental setup -- 9. Results and analysis -- 10. Properties of the proposed predictors -- 11. Conclusions and future directions -- A. Webservers for protein subcellular localization -- B. Support vector machines -- C. Proof of no bias in LOOCV -- D. Derivatives for penalized logistic regression -- Bibliography -- Index |
| Record Nr. | UNINA-9910819391103321 |
|
Wan Shibiao
|
||
| Berlin, Germany ; ; Boston, Massachusetts : , : De Gruyter, , 2015 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Membrane protein purification and crystallization [[electronic resource] ] : a practical guide / / edited by Carola Hunte and Gebhard von Jagow and Hermann Schagger
| Membrane protein purification and crystallization [[electronic resource] ] : a practical guide / / edited by Carola Hunte and Gebhard von Jagow and Hermann Schagger |
| Edizione | [2nd ed.] |
| Pubbl/distr/stampa | Amsterdam ; ; Boston, : Academic Press, c2003 |
| Descrizione fisica | 1 online resource (339 p.) |
| Disciplina | 572/.696 |
| Altri autori (Persone) |
JagowGebhard von
SchäggerHermann |
| Soggetto topico | Membrane proteins - Purification |
| Soggetto genere / forma | Electronic books. |
| ISBN |
1-281-02929-7
9786611029296 0-08-053617-4 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Front Cover; Membrane Protein Purification and Crystallization: A Practical Guide; Copyright Page; Contents; Contributors; Preface; PART I: STRATEGIES AND TECHNIQUES; Chapter 1. Purification Strategies for Membrane Proteins; I. Introduction; II. General Guide for Retaining Catalytic Activity; III. Choice and Sequence of Purification Techniques; IV. Choice of Protein Source, Disruption of Cells, and Preparation of Organelles and Membranes; V. Protein Assay; VI. Solubilization and Stabilization of Membrane Proteins; References
Chapter 2. Techniques and Basic Operations in Membrane Protein Purification I. Introduction; II. Solubilization-Precipitation; III. Concentration of Samples, Exchange of Buffer, and Exchange of Detergent; IV. Chromatographic Techniques; References; Chapter 3. Production and Purification of Recombinant Membrane Proteins; I. Introduction; II. Prokaryotic Expression Systems for Overproduction of Membrane Proteins for Structural Studies; III. Eukaryotic Expression Systems for Overproduction of Membrane Proteins; IV. Use of Fusion Proteins and Affinity Tags for Purification of Membrane Proteins Acknowledgments References; Chapter 4. SDS Electrophoresis Techniques; I. Introduction; II. Choice of Optimal SDS-Page System and Gel Type; III. Tricine-SDS-PAGE; IV. Recovery of Proteins from SDS Gels; V. Processing of Proteins Recovered from Gels for Immunization and Protein Sequencing; VI. Electroblotting; References; Chapter 5. Blue Native Electrophoresis; I. Introduction; II. Techniques; III. Applications; References; Chapter 6. Preparative Isoelectric Focusing; I. Introduction; II. Techniques; III. Application: Purification of Photosystem I; IV. Conclusions; References Chapter 7. Membrane Protein CrystallizationI. Introduction; II. Bottlenecks in Membrane Protein Crystallization; References; PART II: DISCUSSION OF SELECTED ISOLATION PROTOCOLS; Chapter 8. Lipid-Dependent Inactivation and Reactivation of Bovine Complex III; I. Introduction; II. Lipid-Dependent Inactivation and Reactivation; References; Chapter 9. Purification of an Affinity-Epitope Tagged G-Protein Coupled Receptor; I. Introduction; II. Production of Recombinant β2-Adrenergic Receptor in Pichia pastoris; III. Preparation of Pichia pastoris Membranes; IV. Solubilization of Membranes V. Purification of the β2-Adrenergic Receptor VI. Ligand Binding Assays; References; Chapter 10. Purification of NhaA Na+ /H+ Antiporter of Escherichia Coli for 3D or 2D Crystallization; I. Introduction; II. Growth of Escherichia coli for Production of His-Tagged NhaA in a 10 1 Fermenter; III. Isolation of Membranes from Escherichia coli TA16/pAXH Strain; IV. Two-Step Purification of His-Tagged NHaA; V. NhaA Reconstitution into Liposomes and Activity Assay; VI. Dynamic Light Scattering Experiments; Acknowledgments; References; Chapter 11. Purification of the Cytochrome bc1 Complex from Yeast I. Introduction |
| Record Nr. | UNINA-9910458689403321 |
| Amsterdam ; ; Boston, : Academic Press, c2003 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
